There are three KO p300 KO cell lines (p300 KO Clone D10 (HCT116), p300 KO Clone F5 and p300 KO Clone F2) of the p300 WT (HCT116) cell line.
They are an ideal tool for the study of the effects of p300 knockout on the growth potential, adhesion properties and migration of tumour cells in vitro.
The cell lines are derived from human colorectal carcinoma cell line HCT116, are deficient for p300, a transcriptional cofactor involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties.
The p300 KO lines demonstrate an 'aggressive' cancer phenotype in vitro, with loss of cell-cell adhesion, defects in cell-matrix adhesion, and increased migration through collagen matrices. Experiments should be conducted using two of the three KO cell lines. The parental cell line HCT116 will also be required.
Somatic inactivating mutations are associated with several cancers including breast, colorectal and gastric cancers. |
