| Relevance : |
Helper-free packaging cell line & shuttle vector system enabling high-titer production of recombinant retroviral vectors for mammalian gene transfer, with significantly reduced probability of replication-competent retrovirus generation |
| Description : |
WE packaging cell line and pBabe shuttle vector system enabling high-titer production of Moloney murine leukemia retrovirus gene transfer vectors |
| Cell Line Type / Application : |
Viral packaging / production line |
| Species / Origin : |
Mouse; for details of production of WE cell line and pBabe vectors see Morgenstern JP & Land H, Nucleic Acids Research, 1990, v18 pp3587-3596 |
| Growth / Phenotype Keywords : |
Recombinant retroviral production |
| Disease Keywords : |
- |
| Recommended Growth Conditions : |
For recommended growth and recombinant retrovirus production conditions, see Morgenstern JP & Land H, Nucleic Acids Research, 1990, v18 pp3587-3596 |
| References : |
Morgenstern JP & Land H, Nucleic Acids Research, 1990, v18 pp3587-3596  |
| Notes : |
The WE and pBabe recombinant retroviral system utilises shuttle vectors with multiple drug selection markers (the pBabe vector series) for increased flexibility of gene transfer vector design, and a third generation, helper-free packaging cell line (WE) derived from mouse NIH 3T3 cell lines, enabling high-titer production of Moloney murine leukemia virus for use in mammalian gene transfer.
For more retroviral mammalian gene transfer systems, see also;
FLYA13 cell line; FLYRD18 cell line; |
For further information about mouse models from CRT, or to discuss supply or licensing options, please contact us at reagents@cancertechnology.com
Click here for general information on supply, pricing and terms and conditions. |
