| Relevance : |
In vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway |
| Description : |
Panel of IFNg signalling mutant lines (10 lines plus control) each containing a different mutation in the IFN signalling pathway |
| Cell Line Type / Application : |
Knockout panel |
| Species / Origin : |
Human; HT1080 human sarcoma cell lines transfected with a vector encoding a selectable marker regulated by interferon to create the 2fTGH cell line, enabling selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the 2fTGH cell line enabled isolation of 10 IFNg signalling mutants. |
| Growth / Phenotype Keywords : |
Interferon resistance |
| Disease Keywords : |
Immune response / disease; cancer |
| Recommended Growth Conditions : |
Parental 2fTGH and mutant cell lines can be grown in DMEM with 10% FCS |
| References : |
Pellegrini S et al, Molecular and Cellular Biology, 1989, v9 pp4605-4612 
John J et al, Molecular and Cellular Biology, 1991, v11 pp4189-4195
McKendry R et al, PNAS, 1991, v88 pp11455-11459 |
| Notes : |
THe 2fTGH panel is an ideal tool for investigation and comparison of the IFNg signalling pathway.
Chemical mutagenesis of the 2fTGH cell line has generated 10 IFNg signalling mutants;
U4C ()
U2A
U3A
U1A
gamma1
2C4
gamma2A
U6A
U5A
U5A/IFNaR2long |
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