| Antigen: |
Rad51D |
| Relevance: |
RAD51D is a Rad51 paralog.
RAD51 is a eukaryotic homologue of E. coli RecA, a recombinase,
and a component of the homologous recombination DNA
repair pathway. RAD51 forms a nucleoprotein filament
(through binding RAD52 and single stranded DNA that
are exposed following double strand breaks) that initiates
recombination. RAD51D is also a component for the homologous
recombination pathway. |
| Clone: |
Rad51D 5B3/6 |
| Subclass: |
IgG1 |
| Applications: |
Western |
| Immunogen: |
Raised against his-tagged human Rad51D, overexpressed in e.coli and purified on a talon affinity column under denaturing conditions, followed by gel purification on a SDS-Page |
| Reactivity: |
- |
| Species raised in: |
Mouse: Balb/c |
| Fusion partner: |
Sp2/0 Ag.14 |
| Recommended growth conditions: |
DMEM 5% FCS |
| References: |
Masson J-Y et al. 2001.
Genes Dev. 15:3296-3307 
Kawabata M et al. 1999. Biochem. Biophys. Res. Commun.
257:156-162 |
| Notes: |
Does not cross react with
RAD51B, RAD51C, XRCC2 or XRCC3 in Westerns. Does
not recognise Rad51D in HeLa total extracts. |
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