| Antigen: |
Proliferation marker |
| Relevance: |
JC1is a marker of proliferation. It recognises a nuclear antigen distinct from Ki67 present in proliferating cells. It is of especially useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined |
| Clone: |
JC1 |
| Subclass: |
IgG2a |
| Applications: |
Western Blot, Immunohistochemistry (frozen and paraffin sections) |
| Immunogen: |
Pokeweed-stimulated lymphocytes |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Garrido MC, Cordell JL, Becker MHG, Key G, Gerdes J, Jones M, Gatter KC, Mason DY (1992) Monoclonal antibody JC1: New reagent for studying cell proliferation. J Clin Pathol 45:860-865  |
| Notes: |
The antigen recognised recognises bands of 212 and 123 kDa
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
For further information about antibodies from CRT or to discuss sales or licensing options, please contact us at reagents@cancertechnology.com
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