| Antigen: |
Proliferating cell nuclear antigen, (PCNA), also known as cyclin or polymerase delta accessory protein |
| Relevance: |
PCNA, also known as polymerase delta auxiliary protein, is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumour tissue (i.e. relevant to prognosis). |
| Clone: |
PC8 |
| Subclass: |
IgG3 |
| Applications: |
Western blot, Immunohistochemistry (Paraffin embedded), Immunoprecipitation |
| Immunogen: |
Protein A-PCNA fusion obtained from pC2T. |
| Reactivity: |
Human, Yeast (s. Pombe), Insect |
| Species raised in: |
Mouse |
| Fusion partner: |
SP2/0-Ag14 |
| Recommended growth conditions: |
RPMI 1640 + 5% FCS. |
| References: |
Waseem N & Lane DJ. 1990. Cell Sci. 96:121-129  |
| Notes: |
PCNA Epitope also found in Saccharomyces pombe (yeast) and Spodoptera frugiperda(insect) as well as man. Reactive on formalin-fixed paraffin embedded sections. |
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