| Antigen: |
Polo-like-kinase protein;
Detects the mitotic from of plx from xenopus and is
thought to bind to a phosphorylated epitope |
| Relevance: |
Polo-like kinase is a mitotic regulator conserved from yeasts to humans. Many key cell cycle regulators such as p53, cdc25, and cyclin B are directly targeted by polo-like kinase. |
| Clone: |
AZ44 |
| Subclass: |
IgG1 |
| Applications: |
ELISA, Western blot |
| Immunogen: |
baculo expressed Polo Kinase from Xenopus |
| Reactivity: |
Xenopus |
| Species raised in: |
Mouse: Balb/c |
| Fusion partner: |
Sp2/0 Ag.14 |
| Recommended growth conditions: |
DMEM 5% FCS |
| References: |
- |
| Notes: |
Plx is activated by phosphorylation.
AZ44 only blots the Xenopus mitotic form of Plx which
we assume to be due to this antibody recognizing a phosphorylated
epitope. Therefore until I have formally proved that
this antibody recognises a phosphorylated epitope it
is possible that the antibody recognises some other
modification, however the most likely explanation is
that it blots a phosphorylated epitope. Positive control:
xenopus egg extract. Use at 1ug/ml |
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