Polo-like-kinase protein;
recognises Human and Xenopus
Relevance:
Polo-like kinase is a mitotic regulator conserved from yeasts to humans. Many key cell cycle regulators such as p53, cdc25, and cyclin B are directly targeted by polo-like kinase.
Clone:
AZ27
Subclass:
IgG2b
Applications:
ELISA, Western blot
Immunogen:
baculo expressed Polo Kinase from Xenopus
Reactivity:
Human and Xenopus
Species raised in:
Mouse: Balb/c
Fusion partner:
Sp2/0 Ag.14
Recommended growth conditions:
DMEM 5% FCS
References:
-
Notes:
Positive control: Hela cells. 68 kDa MW
Purified antibody is not available, only supernatant. Suggested dilution of supernatant for western blot is 1/10
Legend:Blot of Xenopus egg extracts (Mitotic CSF(3) and intephase (4)) and HeLa (human)(1) and mouse myeloid cell extract (2). Molecular weight markers 120, 98, 68, 57, 43, 32, 18, 12 kD. Note that the phosphorylated form of Polo kinase from the Xenopus mitotic extract migrates slower than the unphosphorylated form present in the interphase extract.
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