| Antigen: |
p53. The epitope probably lies between amino acids 156 and 214 on murine p53. |
| Relevance: |
p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. |
| Clone: |
PAb 240 |
| Subclass: |
IgG1 k |
| Applications: |
Immunoprecipitation, Western blot, FACS, Immunohistochemistry (Paraffin embedded) |
| Immunogen: |
A gel purified p53-b-galactosidase fusion protein containing p53 sequence from amino acids 14-389 (derived from the pSV53C p53 cDNA clone) |
| Reactivity: |
Mammals |
| Species raised in: |
Mouse: Balb/c |
| Fusion partner: |
SP2 |
| Recommended growth conditions: |
RPMI 1640 + 7.5% FCS |
| References: |
Gannon J et al. 1990. EMBO. J. 9:1595-1602. 
Bartek J et al1991. Oncogene. 6:1699-1703 |
| Notes: |
Detection of p53 using
PAb 240: 50% colon cancer sections are positive, 30%
of breast cancer sections and 70% of lung cancer sections.
Normal and premalignant tissue are negative. 50 kDa
MW. Positive control: MDA-MB-231 cell line. |
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