| Antigen: |
Nucleophosmin (N-terminus) |
| Relevance: |
Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. It is also a crucial regulator of p53; it is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. In its cellular structure role, there is evidence suggesting NPM is associated with the centrosome. It is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division) |
| Clone: |
NA24 |
| Subclass: |
IgG1 |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections) |
| Immunogen: |
Nucleophosmin recombinant protein |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Cordell JL, Pulford KAF, Bigerna B, Roncador G, Banham A, Colombo E, Pelicci P-G, Mason DY, Falini B (1999) Detection of normal and chimeric nucleophosmin in human cells. Blood 93:632-642  |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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