| Antigen: |
Cytokeratins of 46, 48, 50, 55, 56, 56.5, 59, 61, 62, 64, 65, 66 and 67 kDa |
| Relevance: |
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue- and differentiation-specific expression profiles |
| Clone: |
K20 |
| Subclass: |
IgG2a, kappa |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections) |
| Immunogen: |
Hair keratin |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Gatter KC, Dunnill MS, Pulford KA, Heryet A, Mason DY (1985) Human lung tumours: a correlation of antigenic profile with histological type. Histopathology 9:805-823 
Pulford KAF, Gatter KC, Mason DY (1985) The characterization of two monoclonal anti-keratin antibodies and their use in the study of epithelial disorders. Histopathology 9:825-840 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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