| Antigen: |
Cytokeratins of 61-62 kDa |
| Relevance: |
Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue- and differentiation-specific expression profiles |
| Clone: |
PK121 |
| Subclass: |
unknown |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections) |
| Immunogen: |
Epidermal keratin |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Gatter KC, Ralfkiaer E, Skinner J, Brown D, Heryet A, Pulford KA, Hou-Jensen K, Mason DY (1985) An immunocytochemical study of malignant melanoma and its differential diagnosis from other malignant tumours. J Clin Pathol 38:1353-1357  |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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