| Antigen: |
Collagen type VII; epitope is in the carboxy terminus |
| Relevance: |
Typed VII Collagen is found in anchoring fibrils at the dermal-epidermal junction. Type VII collagen is defective in Recessive Dystrophic Epidemolysis Bullosa (RDEB). LH7.2 can be used for differentiating invasive from non-invasive melanoma through clear visualisation of appearance and integrity of epidermal basement membrane and diagnosis and antenatal diagnosis of RDEB. |
| Clone: |
LH7.2 |
| Subclass: |
IgG3 |
| Applications: |
Immunohistochemistry(Frozen), Western blot |
| Immunogen: |
Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions. |
| Reactivity: |
- |
| Species raised in: |
Mouse: Balb/c |
| Fusion partner: |
Sp2/0-Ag14 |
| Recommended growth conditions: |
DMEM 5% FCS |
| References: |
Leigh IM et al. 1987. Epithelia. 1:17-29.
Shimizu H et al. 1990. Br. J. Dermatol. 122:577-585  |
| Notes: |
- |
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