| Antigen: |
Biotin |
| Relevance: |
Anti-Biotin antibodies can be used to detect both biotinylated proteins and nucleic acids. Biotin is a water-soluble vitamin, generally classified as a B-complex vitamin. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species |
| Clone: |
BK-1/39 |
| Subclass: |
IgG1 |
| Applications: |
Immunohistochemistry |
| Immunogen: |
Biotin conjugated to keyhole limpet haemocyanin |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
- |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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