| Antigen: |
Adenomatous Polyposis Coli (APC) protein; N-terminal region |
| Relevance: |
The adenomatous polyposis coli tumour suppressor gene is mutated (often deletion of the C-terminal portion of APC) in the inherited disease, familial adenomatous polyposis (FAP), and over 80% of colorectal cancers. Ali 12-28 can be used for APC expression and detection of APC mutations. |
| Clone: |
Ali 12-28 |
| Subclass: |
IgG1 |
| Applications: |
Western blot,Immunohistochemistry (Acetone Fixed, Frozen), ELISA, Immunoprecipitation |
| Immunogen: |
N-terminal APC-MBP fusion protein (first 1.3 kb of APC which includes the first 433 aa and the first 9 exons of the gene). |
| Reactivity: |
- |
| Species raised in: |
Mouse: Balb/c |
| Fusion partner: |
- |
| Recommended growth conditions: |
DMEM + 5% FCS |
| References: |
Efstathiou JA et al. 1998.
Proc. Natl. Acad. Sci. USA. 95(6):3122-7  |
| Notes: |
Use: 4 µg/ml for western blotting; 20 µg/ml for immunofluorescence. positive lysate: cancer cell lines SW480 / LOVO / DLD1 / SW837. 310 kDa MW. |
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Please note that CRT supplies to distributors only. However, we are happy to direct researchers to the appropriate commercial party to obtain antibodies within our portfolio. Click here to view disclaimer information. |
