| Antigen: |
Spastin |
| Relevance: |
Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutant forms of spastin are generally found throughout the cytoplasm rather then within the nucleus. |
| Clone: |
Sp 6C6 |
| Subclass: |
IgG2a |
| Applications: |
Western blot, ELISA, Immunofluoresence |
| Immunogen: |
recombinant human spastin |
| Reactivity: |
Human, mouse, rat |
| Species raised in: |
Balb/c mouse |
| Fusion partner: |
Sp2/0 Ag.14 |
| Recommended growth conditions: |
DMEM 5% FCS |
| References: |
Salinas S et al. 2005 J Neurochem. 95(5):1411-20  |
| Notes: |
Positive control: total HeLa extract or rat brain
Suggested dilution for WB: 1:500
There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein. 52kDa is the major band. |
| Image: |
WB of rat brain synaptosome |
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