S-100 clone S1-61

Anti-S-100 Antibody Profile

Antigen:	S-100
Relevance:	S-100 protein is an acidic protein antigen present on certain cells and useful as a marker in anatomic pathology. The name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. The protein binds calcium and is structurally similar to calmodulin. The function of S-100 is unknown. S-100 is normally present in cells derived from the neural crest (Schwann cells, melanocytes, and glial cells), chondrocytes, adipocytes, myoepithelial cells, macrophages, Langerhans cells, and dendritic cells. It may be present in some breast epithelial cells
Clone:	S1-61
Subclass:	IgG1
Applications:	Immunohistochemistry (frozen and paraffin sections)
Immunogen:	S-100 protein conjugated to methylated BSA
Reactivity:	Human
Species raised in:	Mouse
Fusion partner:	NS-1
Recommended growth conditions:	RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References:	Vanstapel M-J, Peeters B, Cordell J, Heyns W, de Wolf-Peeters C, Desmet V, Mason D (1985) Production of monoclonal antibodies directed against antigenic determinants common to the alpha- and beta-chain of bovine brain S-100 protein. Lab Invest 52:232-238 Vanstapel M-J, Gatter KC, de Wolf-Peeters C, Mason DY, Desmet VD (1986) New sites of human S-100 immunoreactivity detected with monoclonal antibodies. Am J Clin Pathol 85:160-168
Notes:	Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well

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