| Antigen: |
IgM |
| Relevance: |
For use as a primary or secondary antibody |
| Clone: |
R1/69 |
| Subclass: |
IgG1 |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections) |
| Immunogen: |
Human myeloid cells |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Erber WN, Falini B, Ghosh AK, Moir DJ, Mason DY (1983) Immuno-alkaline phosphatase labelling of haematological samples with monoclonal antibodies. In Feldman G (ed) Proc 2nd Int Symp Immunoenzymatic Techniques, Elsevier, North Holland, p 29-40 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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