Glycophorin A clone JC159

Anti-Glycophorin A Antibody Profile

Antigen:	Glycophorin A
Relevance:	Glycophorin A (GpA) is a 131 amino acid sialoglycoprotein that spans the membrane once and presents its amino-terminal end at the extra-cellular surface of the human red blood cell and precursor cells. It has been shown that glycophorins act as the receptor for Sandei virus and parvovirus
Clone:	JC159
Subclass:	IgG1
Applications:	Immunohistochemistry (frozen and paraffin sections), FACS
Immunogen:	Membrane preparation from splenic hairy cell leukaemia cells
Reactivity:	Human
Species raised in:	Mouse
Fusion partner:	NS-1
Recommended growth conditions:	RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References:	Erber WN, McLachlan J, Cordell JL, Turley H, Reid M, Mason DY (1991) A new monoclonal antibody (JC159) that detects glycophorin A for the diagnosis of erythroleukaemia. Hematology Reviews 5:113-120 Sadahira Y et al. 1999. J Clin Pathol. 52(12): 919-21
Notes:	Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well

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