| Antigen: |
CD8 (Leu-2) |
| Relevance: |
CD8 is a T cell co-receptor that recognises, together with the T cell receptor, MHC class I molecules. CD8 is present on human suppressor / cytotoxic T cells which make up 30% of circulating T cells. C8/144B can be used to detect these cells in tissue sections and also their neoplastic counterparts. |
| Clone: |
C8/144B |
| Subclass: |
IgG1 |
| Applications: |
Western Blot, Immunohistochemistry (frozen and paraffin), Immunoprecipitation |
| Immunogen: |
Synthetic peptide ( the 13 c-terminal amino-acids of the cytoplasmic domain of human CD8a) |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Mason DY, Cordell JL, Gaulard P, Tse AGD, Brown MH (1992) Immunohistological detection of human cytotoxic/suppressor T cells using antibodies to a CD8 peptide sequence. J Clin Pathol 45:1084-1088  |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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