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Anti-Transferrin receptor Antibody Profile

Antigen: Transferrin receptor (CD71)
Relevance: The transferrin receptor is a glycoprotein composed of disulfide-linked polypeptide chains.  It is ubiquitously distributed on the cell surface of actively growing human cells.  Q1/71 can be used as a marker of proliferation
Clone: Q1/71
Subclass: IgG1
Applications: Western blot, Immunohistochemistry (frozen sections), FACS
Immunogen: Foetal liver cells
Reactivity: Human
Species raised in: Mouse
Fusion partner: NS-1
Recommended growth conditions: RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References: CD Guide for CD71 (1989) In Knapp W, et al (eds) Leucocyte Typing IV, Oxford University Press, Oxford, New York and Tokyo, p 1091
Notes: Please note this cell line has not been weaned out of HAT.  Ultroser G can be used at 1% if the cells are not growing well

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