CD61 clone Y2/51

Anti-CD61 Antibody Profile

Antigen:	CD61 (Integrin b3; GPIIIa)
Relevance:	CD61 is expressed by megakaryocytes, platelets and osteoclasts. Y2/51 is widely used as a marker of megakaryocytes in bone marrow trephines
Clone:	Y2/51
Subclass:	IgG1
Applications:	Western blot, Immunohistochemistry (frozen and paraffin sections), FACS
Immunogen:	Phytohaemagglutin-stimulated peripheral blood cells
Reactivity:	Human
Species raised in:	Mouse
Fusion partner:	NS-1
Recommended growth conditions:	RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References:	Nachman RL, Leung LL (1982) Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen. J Clin Invest 69:263-269 Vinci G, Tabilio A, Deschamps JF, Van Haeke D, Henri A, Guichard J, Tetteroo P, Lansdorp PM, Hercend T, Vainchenker W, et al (1984) Immunological study of in vitro maturation of human megakaryocytes. Br J Haematol 56:589-605 Gatter KC, Cordell JL, Turley H, Heryet A, Kieffer N, Anstee DJ, Mason DY (1988) The immunohistological detection of platelets, megakaryocytes and thrombi in routinely processed specimens. Histopathology 13:257-267
Notes:	Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well

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