| Antigen: |
CD50 (ICAM-3) |
| Relevance: |
ICAM-3 is the major ligand for LFA-1 (CD11a/CD18) in the resting state. ICAM-3 may play a key role in initiating immune responses |
| Clone: |
By44 |
| Subclass: |
IgG1, kappa
|
| Applications: |
Immunohistochemistry (frozen sections), Immunoprecipitation, FACS |
| Immunogen: |
B cell lymphoma cells |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Doussis-Anagnostopoulou I, Kaklamanis L, Cordell J, Jones M, Turley H, Pulford K, Simmons D, Mason D, Gatter K (1993) ICAM-3 expression on endothelium in lymphoid malignancy. Am J Pathol 143:1040-1043 
Cordell JL, Pulford K, Turley H, Jones M, Micklem K, Doussis IA, Tyler X, Mayne K, Gatter KC, Mason DY (1994) Cellular distribution of human leucocyte adhesion molecule ICAM-3. J Clin Pathol 47:143-147 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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