CD45RB clone PD7/26

Anti-CD45RB Antibody Profile

Antigen:	CD45RB
Relevance:	CD45 is a transmembrane tyrosine phosphatase that is present on all leukocytes. CD45 regulates the threshold of T cell antigen receptor (TCR) signaling through dephosphorylation of protein tyrosine kinases (e.g. Lck and Fyn). CD45RB is expressed on peripheral B cells, T cytotoxic cells and CD4+ T cells. It has a role in cell signalling regulation through the activation of the Src family of tyrosine kinases. PD7/26 is widely used, in conjunction the CD45 antibody 2B11, to detect white cell-derived tumours in paraffin sections
Clone:	PD7/26
Subclass:	IgG1
Applications:	Western blot, Immunohistochemistry (frozen and paraffin sections), FACS
Immunogen:	Human PBL maintained in T-cell growth factor
Reactivity:	Human
Species raised in:	Mouse
Fusion partner:	NS-1
Recommended growth conditions:	RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT
References:	Gatter KC, Cordell JL, Falini B, Ghosh AK, Heryet A, Nash JRG, Pulford KAF, Moir DJ, Erber WN, Stein H, Mason DY (1983) Monoclonal antibodies in diagnostic pathology: techniques and applications. J Biol Response Mod 2:369-395 Warnke RA, Gatter KC, Falini B, Hildreth P, Woolston R-E, Pulford K, Cordell JL, Cohen B, de Wolf-Peeters C, Mason DY (l983) Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med 309:1275-1281
Notes:	Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well

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