| Antigen: |
CD32 (Fc gamma receptor II) |
| Relevance: |
Human CD32 exists in at least six isoforms originating from three different genes (Fc gamma RII A, B, and C). The CD32 molecule is a low affinity receptor for immune complexed IgG and has signal-transducing capabilities involved with humoral and cell-mediated immune responses. It is expressed by Monocytes, granulocytes, B cells, eosinophils |
| Clone: |
KB61 |
| Subclass: |
IgG1, kappa |
| Applications: |
Immunohistochemistry (frozen sections), Immunoprecipitation, FACS |
| Epitope: |
Cell surface |
| Immunogen: |
Hairy cell leukaemia cells |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Pulford K, Ralfkiaer E, MacDonald SM, Erber WN, Falini B, Gatter KC, Mason DY (1986) A new monoclonal antibody (KB61) recognizing a novel antigen which is selectively expressed on a subpopulation of human B lymphocytes. Immunology 57:71-76 
Greenman J, Tutt AL, George AJ, Pulford KA, Stevenson GT, Glennie M J (1991) Characterization of a new monoclonal anti-Fc gamma RII antibody, AT10, and its incorporation into a bispecific F(ab')2 derivative for recruitment of cytotoxic effectors. Mol Immunol 28:1243-1254 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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