| Antigen: |
CD31
(Platelet Endothelail CAM-1, Platelet GPIIa) |
| Relevance: |
PE-CAM1 (CD31) is a member of the immunoglobulin (Ig) superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, particular T-cell subsets, and is also a major constituent of the endothelial cell intercellular junction. PECAM1 is implicated in several functions, including transendothelial migration of leukocytes, angiogenesis, and integrin activation. JC70 is a good marker of endothelial cells in paraffin sections |
| Clone: |
JC70 |
| Subclass: |
IgG1 |
| Applications: |
Western blot, Immunohistochemistry (frozen and paraffin sections), FACS |
| Epitope: |
Cell surface |
| Immunogen: |
Cell membrane preparation from the spleen of a patient with hairy cell leukaemia |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Parums DV, Cordell JL, Micklem K, Heryet AR, Gatter KC, Mason DY (1990) JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol 43:752-757 
Muller WA (1997) CD31 workshop panel report. In Kishimoto T, et al (eds) Leucocyte Typing VI, Garland Publishing Inc., New York and London, p 362-364 |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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