| Antigen: |
CD16 (low affinity Fc gamma receptor III, FcgRIII, Leu II) |
| Relevance: |
FcgRIII mediates phagocytosis and antibody-dependent cell-mediated cytotoxicity. It is expressed on neutorphils, NK cells and macrophages |
| Clone: |
DJ130c |
| Subclass: |
IgG1 |
| Applications: |
Western blot, Immunohistochemistry (paraffin and frozen sections) |
| Immunogen: |
CD34 antigen (unrelated) |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Schmidt RE (1993) CD16 cluster workshop report. In Schlossman SF, et al (eds) Leucocyte Typing V, Vol 1, Oxford University Press, Oxford, New York and Tokyo, p 805-806
Zhu X, Hamann KJ, Munoz NM, Rubio N, Mayer D, Hernrreiter A, Leff AR (1998) Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human esinophils. J Immunol 161:2574-2579  |
| Notes: |
Epitope is on the first membrane-distal domain of CD16. DJ130c recognises both CD16-A and CD16-B
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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