| Antigen: |
CD15 |
| Relevance: |
CD15 is a branched pentasaccharide found on neutrophils, eosinophils and monocytes. CD15 is distributed abnormally in myeloid leukeamias. CD15 is commonly used in the diagnosis of Hodgkin’s disease. It can also be used for analysis of myeloid leukaemias and studies of myeloid differentiation |
| Clone: |
By87a |
| Subclass: |
IgM |
| Applications: |
Western blot, Immunohistochemistry (Frozen and paraffin sections), FACS |
| Immunogen: |
B cell lymphoma cells |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Dorfman RF, Gatter KC, Pulford KAF, Mason DY (1986) An evaluation of the utility of anti-granulocyte and anti-leukocyte monoclonal antibodies in the diagnosis of Hodgkin's disease. Am J Pathol 123:508-519  |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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