| Antigen: |
Nav 1.5 (Voltage-gated Socium Channel Nav 1.5) |
| Relevance: |
Voltage-gated sodium channesl (VGSCs) mediate regenerative cell membrane depolarization and conduction of electrical signalling in nerves and muscles. Expression is also detected in lymphocytes, glia, and fibroblasts. VGSC proteins are comprised of a core alpha-subunit, together with one or more additional regulatoy beta-subunit. Expression has been detected in a number of tumour cell lines and has been implicated in the metastatic behaviour of breast and prostate cancer cells. |
| Clone: |
4G8:1G7 |
| Subclass: |
IgM |
| Applications: |
Western blot, Immunocytochemistry |
| Immunogen: |
Peptide from an extracellular domain of Nav1.5 |
| Reactivity: |
Human |
| Species raised in: |
Mouse: CD1 |
| Fusion partner: |
|
| Recommended growth conditions: |
DMEM + 5% FCS |
| References: |
|
| Notes: |
Positive control: cell lines over expressing Nav1.5, e.g., Nav 1.5-HEK293 Recombinant cell line from Upstate. Western blotting and Immunocytochemistry carried out at 2ug/ml and 10ug/ml, respectively. |
 Figure A: Plasma membrane localisation of Nav1.5 identified by 4G8:1G7 detected by anti-mouse Alexa Fluor-488 conjugated antibody; Figure B, DAP1 co-staining of cell nuclei |
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