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Anti Lewis A-Antibody Profile

Antigen: Lewis A
Relevance: Lewis a antigen is expressed on colonic mucus, epithelial cells and cell membranes. PR 4D2 is useful for histological diagnosis of colon disorders. Wide reactivity with bowel, small intestine, stomach and colo-rectal tumours.
Clone: PR 5C5
Subclass: IgG1
Applications: ELISA
Immunogen: Mucosal scrapings and membrane preparations from normal colorectal epithelium and possibly booster inoculations with the colon carcinoma cell line HT29.
Reactivity:  
Species raised in: Balb/c
Fusion partner: P3/NS1/1-Ag4-1
Recommended growth conditions: DMEM 5% FCS
References: Richman P.R. and Bodmer W.F. Int. J. Cancer 39: p317-328 (1987)
Notes: Reactive with formalin fixed tissues

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