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Anti-poliovirus 3 Antibody Profile

Antigen: Poliovirus type 3
Clone: 204, 520, 495, 889
Subclass:

IgM: 495, 889

IgG: 204

Unknwon: 520

Applications:

In vitro potency assays of inactivated poliovaccines (520)

Intertypic differentiation of poliovirus strains in neutralisation assays  (495, 889)

Assays of the virus content of trivalent oral poliovaccines (495)

Serotyping polioviruses by direct immunofluorescence (204)

Immunogen:

Poliovirus type 3 Sabin vaccine virus (clones 520, 495)

Trypsin treated poliovirus type 3 Sabin vaccine virus (clone 889)

Poliovirus type 3 Leon from Sabin (clone 204)

Reactivity: Poliovirus
Species raised in: Mouse: Balb/C
Fusion partner:

P3.X63-Ag8 (for clone 204)

NSO (for clones 520, 495, 889)

Recommended growth conditions:

RPMI 1640

10% FCS

0.3% L-glutamine

1 x hypoxanthine-thymidine
References: -
Notes: All clones are broadly reactive with type 3 poliovirus strains except clone 889 which reacts only with virus strains derived from type 3 Sabin vaccine virus

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