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Anti-poliovirus 2 Antibody Profile

Antigen: Poliovirus type 2
Clone: 267, 1037, 1050, 697, 1233
Subclass:

IgG3: 267, 697

IgG2a: 1037, 1050

IgM: 1233

Applications:

In vitro potency assays of inactivated poliovaccines (1037, 1050) Intertypic differentiation of poliovirus strains in neutralisation assays  (697, 1233)

Assays of the virus content of trivalent oral poliovaccines (267)

Serotyping polioviruses by direct immunofluorescence (267)

Immunogen:

Poliovirus 2 Sabin vaccine virus (clones 267, 697)

Trypsin treated poliovirus 2 Sabin vaccine virus (1037, 1050, 1233)

Reactivity: Poliovirus
Species raised in: Mouse: Balb/C
Fusion partner:

P3.X63-Ag8 (for clone 267)

NSO (for clones 1037, 1050, 697, 1233)

Recommended growth conditions:

RPMI 1640

10% FCS

0.3% L-glutamine

1 x hypoxanthine-thymidine
References: -
Notes: All clones are broadly reactive with type 2 poliovirus strains except clone 1233 which reacts only with virus strains derived from type 2 Sabin vaccine virus

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