| Antigen: |
MDM2 phospho specific
(serine 186) |
| Relevance: |
MDM2's principal function
is the ubiquitination and degradation of p53 tumour
suppressor protein, promoting progression through the
cell cycle and cell survival. It is over-expressed
in many tumours. |
| Clone: |
2G2 |
| Subclass: |
IgG1 |
| Applications: |
Western blot |
| Immunogen: |
Peptide QRKRHKS(phos)DSIS. S(phos) represents a phosphorylated serine. This peptide represents the portion of MDM2 around serine 186 |
| Reactivity: |
Human |
| Species raised in: |
Mouse: balb/C |
| Fusion partner: |
Sp2 |
| Recommended growth conditions: |
DMEM + 5% FCS |
| References: |
- |
| Notes: |
Phosphorylation at serine
166 and 186 is necessary for MDM2's translocation into
the nucleus, thereby facilitating interaction with p53.
Positive control: breast carcinoma. Western blots at around 90kDa |
 |
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