| Antigen: |
Alkaline phosphatase (calf intestine) |
| Relevance: |
Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. AP-7/6/7 can be used for IHC and APAAP immuno-alkaline phosphatase technique |
| Clone: |
AP-7/6/7 |
| Subclass: |
IgG1 |
| Applications: |
Immunohistochemistry and APAAP immuno-alkaline phosphatase technique |
| Immunogen: |
Calf intestinal alkaline phosphatase |
| Reactivity: |
Calf |
| Species raised in: |
Mouse |
| Fusion partner: |
NS-1 |
| Recommended growth conditions: |
RPMI 1640 + 10% FCS + penicillin (100U/ml) + streptomycin (100mg/l) + glutamine (2mM) + HAT |
| References: |
Cordell JL, Falini B, Erber WN, Ghosh AK, Abdulaziz Z, MacDonald S, Pulford KAF, Stein H, Mason DY (1984) Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem 32:219-229.  |
| Notes: |
Please note this cell line has not been weaned out of HAT. Ultroser G can be used at 1% if the cells are not growing well |
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