| Antigen: |
Ataxia telangiesctasia mutated (ATM) protein |
| Relevance: |
The ATM protein is a member of the phosphatidylinositol-3 kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. It is thought that activation of the ATM kinase by autophosphorylation is the initiating event of cellular responses to irradiation. The classic form of ataxia telangiectasia, an autosomal recessive cerebellar ataxia, results from the presence of two truncating ATM mutations, leading to total loss of the ATM protein. |
| Clone: |
ATM 11G12 |
| Subclass: |
IgG1 |
| Applications: |
Western blot, Immunoprecipitation, Immunofluorescence |
| Immunogen: |
aa 992-1144 of ATM fusion protein |
| Reactivity: |
Human |
| Species raised in: |
Mouse |
| Fusion partner: |
sp2/0 Ag14 |
| Recommended growth conditions: |
DMEM 5% FCS |
| References: |
Starczynski J et al. 2003. American J Pathology. 163; 423-432  |
| Notes: |
dilution suggestion: 1:500 dilution in 5% milk/PBST and incubated overnight at 4 degrees |
| Image: |
Western blot using the ATM 11G12 monoclonal Ab. There are four lanes (1-4). Each lane contains a lysate of a lymphoblastoid cell line (LCL) that we derived from either patient or control blood samples. In lanes 1 and 4, respectively, are lysates of two LCLs from two normals showing ATM. In lane 2 is lysate from a classical A-T patient with two truncating ATM mutations showing no ATM protein expression; lane 3 is lysate from an A-T patient carrying a splicing mutation that results in expression of ~5% of normal ATM. |
| |
 |
For further information about antibodies from CRT or to discuss sales or licensing options, please contact us at reagents@cancertechnology.com
Click here to view Notes and Disclaimer information |
